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raw blue isg reporter cells  (InvivoGen)


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    Structured Review

    InvivoGen raw blue isg reporter cells
    Raw Blue Isg Reporter Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raw+blue+isg/RAW-Blue+cells/pm39933120-137-0-8
    Average 96 stars, based on 291 article reviews
    raw blue isg reporter cells - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Bacillus Calmette-Guérin Overexpressing an Endogenous Stimulator of Interferon Genes Agonist Provides Enhanced Protection Against Pulmonary Tuberculosis
    Article Snippet: .. RAW-Blue ISG (InvivoGen) reporter cells, derived from the murine RAW 264.7 macrophage cell line by stably integrating an IRF-inducible secreted embryonic alkaline phosphatase (SEAP) reporter construct, were used for IRF activation assay. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: For immunofluorescence and western blot detection of LC3, at 6 h after infection macrophage cells were washed thoroughly and either fixed in 4% paraformaldehyde in PBS followed by immunofluorescence staining or lysates were prepared in RIPA buffer (Cell Signaling Technologies) for western blotting. .. RAW-Blue ISG and RAW-Lucia ISG or RAW-Lucia ISG-KO-STING (InvivoGen) cells were derived from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) and luciferase reporter constructs, respectively. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: For immunofluorescence and western blot detection of LC3, at 6 h after infection macrophage cells were washed thoroughly and either fixed in 4% paraformaldehyde in PBS followed by immunofluorescence staining or lysates were prepared in RIPA buffer (Cell Signaling Technologies) for western blotting. .. RAW-Blue ISG and RAW-Lucia ISG or RAW-Lucia ISG-KO-STING (InvivoGen) cells were derived from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) and luciferase reporter constructs, respectively. ..

    Stable Transfection:

    Article Title: Bacillus Calmette-Guérin Overexpressing an Endogenous Stimulator of Interferon Genes Agonist Provides Enhanced Protection Against Pulmonary Tuberculosis
    Article Snippet: .. RAW-Blue ISG (InvivoGen) reporter cells, derived from the murine RAW 264.7 macrophage cell line by stably integrating an IRF-inducible secreted embryonic alkaline phosphatase (SEAP) reporter construct, were used for IRF activation assay. ..

    Construct:

    Article Title: Bacillus Calmette-Guérin Overexpressing an Endogenous Stimulator of Interferon Genes Agonist Provides Enhanced Protection Against Pulmonary Tuberculosis
    Article Snippet: .. RAW-Blue ISG (InvivoGen) reporter cells, derived from the murine RAW 264.7 macrophage cell line by stably integrating an IRF-inducible secreted embryonic alkaline phosphatase (SEAP) reporter construct, were used for IRF activation assay. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: For immunofluorescence and western blot detection of LC3, at 6 h after infection macrophage cells were washed thoroughly and either fixed in 4% paraformaldehyde in PBS followed by immunofluorescence staining or lysates were prepared in RIPA buffer (Cell Signaling Technologies) for western blotting. .. RAW-Blue ISG and RAW-Lucia ISG or RAW-Lucia ISG-KO-STING (InvivoGen) cells were derived from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) and luciferase reporter constructs, respectively. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: For immunofluorescence and western blot detection of LC3, at 6 h after infection macrophage cells were washed thoroughly and either fixed in 4% paraformaldehyde in PBS followed by immunofluorescence staining or lysates were prepared in RIPA buffer (Cell Signaling Technologies) for western blotting. .. RAW-Blue ISG and RAW-Lucia ISG or RAW-Lucia ISG-KO-STING (InvivoGen) cells were derived from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) and luciferase reporter constructs, respectively. ..

    Activation Assay:

    Article Title: Bacillus Calmette-Guérin Overexpressing an Endogenous Stimulator of Interferon Genes Agonist Provides Enhanced Protection Against Pulmonary Tuberculosis
    Article Snippet: .. RAW-Blue ISG (InvivoGen) reporter cells, derived from the murine RAW 264.7 macrophage cell line by stably integrating an IRF-inducible secreted embryonic alkaline phosphatase (SEAP) reporter construct, were used for IRF activation assay. ..

    Luciferase:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: For immunofluorescence and western blot detection of LC3, at 6 h after infection macrophage cells were washed thoroughly and either fixed in 4% paraformaldehyde in PBS followed by immunofluorescence staining or lysates were prepared in RIPA buffer (Cell Signaling Technologies) for western blotting. .. RAW-Blue ISG and RAW-Lucia ISG or RAW-Lucia ISG-KO-STING (InvivoGen) cells were derived from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) and luciferase reporter constructs, respectively. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: For immunofluorescence and western blot detection of LC3, at 6 h after infection macrophage cells were washed thoroughly and either fixed in 4% paraformaldehyde in PBS followed by immunofluorescence staining or lysates were prepared in RIPA buffer (Cell Signaling Technologies) for western blotting. .. RAW-Blue ISG and RAW-Lucia ISG or RAW-Lucia ISG-KO-STING (InvivoGen) cells were derived from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible secreted embryonic alkaline phosphatase (SEAP) and luciferase reporter constructs, respectively. ..

    Knock-Out:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW 264.7-derived macrophages such as RAW-Blue ISG, RAW-Lucia ISG, RAW-Lucia ISG-KO-STING (Sting knockout cells), human monocyte THP-Dual, THP1-Blu ISG-KD-STING cells (all from InvivoGen) and primary BMDMs and BMDCs from C57BL/6J and cGAS-KO mouse were used for in vitro experiments. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. Both E. coli and mycobacterial strains were grown from frozen glycerol stocks stored at −70° C. Murine macrophage cells J774.1, RAW 264.7—derived macrophages such as RAW-Blue ISG, RAW-Lucia ISG, RAW Lucia ISG-KO-STING (Sting knockout cells), human monocyte THP1-Dual, THP1-Blu ISG-KD-STING cells (all from InvivoGen) and primary BMDMs and BMDCs from C57BL/6J and cGAS-KO mouse were used for in vitro experiments. ..

    In Vitro:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW 264.7-derived macrophages such as RAW-Blue ISG, RAW-Lucia ISG, RAW-Lucia ISG-KO-STING (Sting knockout cells), human monocyte THP-Dual, THP1-Blu ISG-KD-STING cells (all from InvivoGen) and primary BMDMs and BMDCs from C57BL/6J and cGAS-KO mouse were used for in vitro experiments. ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. Both E. coli and mycobacterial strains were grown from frozen glycerol stocks stored at −70° C. Murine macrophage cells J774.1, RAW 264.7—derived macrophages such as RAW-Blue ISG, RAW-Lucia ISG, RAW Lucia ISG-KO-STING (Sting knockout cells), human monocyte THP1-Dual, THP1-Blu ISG-KD-STING cells (all from InvivoGen) and primary BMDMs and BMDCs from C57BL/6J and cGAS-KO mouse were used for in vitro experiments. ..

    Transfection:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Sequencing:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Control:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Plasmid Preparation:

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..

    Article Title: Methods of treating cancer using bacteria expressing c-di-AMP
    Article Snippet: .. RAW Blue ISG (InvivoGen) cells were transfected with a pool of five lentiviral vectors carrying a target gene sequence for DDX41 or a control plasmid (pLKO.1) (Thermo Scientific). ..



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